Enzymatic synthesis of 7-aminocephalosporanic acid(7-ACA).

Preparation of 7-aminocephalosporanic acid and 6-aminopenicillanic acid.

Penicillin acylase-catalyzed synthesis of N-bromoacetyl-7-aminocephalosporanic acid, the key intermediate for the production of cefathiamidine

BACKGROUND Enzymatic approaches have become promising alternatives to chemical methods for the production of semi-synthetic β-lactam antibiotics. In this work, enzymatic synthesis of N-bromoacetyl-7-aminocephalosporanic acid (N-bromoacetyl-7-ACA), the key intermediate for the production of cefathiamidine, was reported for the first time. RESULTS Of the immobilized penicillin acylases (PAs) te...

Possibilities to acylate 7-aminocephalosporanic acid to obtain some cephalosporins.

AIM Acylation of 7-aminocephalosporanic acid with an adequate acyl chloride in order to obtain cefotaxime sodium salt. MATERIAL AND METHODS Cefotaxime sodium salt was synthesized by acylating 7-amino cephalosporanic acid with 2-[2'-chloracetamidothiazole-4-yl]-2-(syn)-methoxy-imino acetic chloride in four steps. The melting point was determined, and IR spectral analysis and elemental analysis...

(R)-α-Aminoadipic Acid: A Versatile Precursor for the Synthesis of D-Amino Acids

The ready accessibility of (R)- α -aminoadipic acid by enzymatic cleavage of cephalosporin C (CephC) in the production of 7-aminocephalosporanic acid (7-ACA) on a large scale makes it a favorable chiral pool building block for the synthesis of unusual amino acids. A route for the synthesis of C-5-alkenyl and C-6-alkylidene derivatives of (R)-pipecolic acid is described which utilizes (R)- α -am...

Enzymatic and chemo-enzymatic synthesis of carbohydrates

Carbohydrate-mediated biochemical recognition processes are potential targets for drug development. The synthesis of complex carbohydrates and related structures, however, has been a difficult task, especially from practical point of view. New solutionand solidphase methods based on enzymatic and a combined enzymatic and chemical procedure have recently been developed for the practical synthesi...